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Signal Transduction Cytoskeleton / ECM Cytoskeleton Intermediate Filaments Class I Cytokeratins

Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)

Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [SPM270] to Cytokeratin 7 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt, WB, ICC
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free
    See all Cytokeratin 7 primary antibodies
  • Description

    Mouse monoclonal [SPM270] to Cytokeratin 7 - BSA and Azide free
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cyt, WB, ICCmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human Cytokeratin 7. From OTN 11, ovarian carcinoma cell line.
    Database link: P08729

  • Positive control

    • Lung, squamous cell carcinoma tissue; HeLa cells; Carcinoma of ovary, cervix, or breast

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Constituent: 100% PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from bioreactor concentrate.
  • Clonality

    Monoclonal
  • Clone number

    SPM270
  • Isotype

    IgG1
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Intermediate Filaments
    • Class I
    • Cytokeratins

Images

  • Western blot - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
    Western blot - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
    Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526) at 2 µg/ml + HeLa cells

    Predicted band size: 51 kDa

  • Immunocytochemistry - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
    Immunocytochemistry - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)

    Immunocytochemistry analysis of HeLa cells labelling Cytokeratin 7 with ab212526 at 2 µg/mL. Goat Anti-Mouse IgG-CF448 (green) was used as the secondary antibody (green). Cells were counterstained with reddot (red).

  • Flow Cytometry - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
    Flow Cytometry - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)

    Flow Cytometric analysis of trypsinised MeOH-fixed HeLa cells labeling Cytokeratin 7 with ab212526 followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [SPM270] - BSA and Azide free (ab212526)

    Immunohistochemical analysis of paraffin embedded human Lung, squamous cell carcinoma tissue labeling Cytokeratin 7 with ab212526 at 0.5 µg/mL.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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