Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)
![Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)](https://www.abcam.com/ps/products/212/ab212547/Images/ab212547-299257-anti-cytokeratin-14-antibody-ll002-immunohistochemistry.jpg "Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)")
Key features and details
- Mouse monoclonal [LL002] to Cytokeratin 14 - BSA and Azide free
- Suitable for: ICC, IHC-P
- Knockout validated
- Reacts with: Human
- Isotype: IgG3
Overview
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Product name
Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free
See all Cytokeratin 14 primary antibodies -
Description
Mouse monoclonal [LL002] to Cytokeratin 14 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human Cytokeratin 14 (C terminal). (15 amino acid peptide).
Database link: P02533 -
Positive control
- IHC-P: Human prostate tissue. ICC: A431 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
ab212547 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
LL002 -
Isotype
IgG3 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)
Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 14 using ab212547 at 1 µg/ml in immunohistochemical analysis.
This IHC-P data was generated using the same anti-Cytokeratin 14 antibody clone, LL002, in a different buffer formulation (cat# ab7800).
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![Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)](https://www.abcam.com/ps/products/212/ab212547/Images/ab212547-3-anti-KRT14-antibody-LL002-immunocytochemistry-immunofluorescence-A431-human.jpg)
Immunocytochemistry - Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)This data was developed using the same antibody clone in a different buffer formulation (ab7800). ab7800 staining KRT14 in wild-type A431 cells (top panel) and KRT14 knockout A431 cells (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7800 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)](https://www.abcam.com/ps/products/212/ab212547/Images/ab212547-299256-anti-cytokeratin-14-antibody-ll002-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [LL002] - BSA and Azide free (ab212547)Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 14 using ab212547 at 1 µg/ml in immunohistochemical analysis.
This IHC-P data was generated using the same anti-Cytokeratin 14 antibody clone, LL002, in a different buffer formulation (cat# ab7800).
