Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17350] to Cytokeratin 14 - BSA and Azide free
  • Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free
    See all Cytokeratin 14 primary antibodies

  • Description

    Rabbit monoclonal [EPR17350] to Cytokeratin 14 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, Flow Cyt (Intra)more details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human skin and squamous cell carcinoma of cervix tissues; Mouse and rat skin tissues. ICC/IF: PC-12 cells and wild-type A431 cells. Flow Cyt (intra): PC-12 cells.

  • General notes

    ab220818 is the carrier-free version of ab181595.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Flow Cytometry - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling Cytokeratin 14 with purified ab181595 at 1/190 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma cells) labeling Cytokeratin 14 with ab181595 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on PC-12 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls:-
    -ve control 1: ab181595 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Immunohistochemical analysis of paraffin-embedded Mouse skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Immunohistochemical analysis of paraffin-embedded Human squamous cell carcinoma of cervix tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Squamous carcinoma cells show strong staining. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

    Immunohistochemical analysis of paraffin-embedded Human skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counter stained with Hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP Polymer for Rabbit/Mouse IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181595).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)
    Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (ab220818)

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