HRP Anti-Cytokeratin 14 antibody [EP1612Y] (ab192081) at 1/5000 dilution + A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 52 kDa
Observed band size: 52 kDa


Exposure time: 90 seconds

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab192081 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

IHC image of Cytokeratin 14 staining in human skin formalin fixed paraffin embedded tissue section*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30 mins. The section was incubated with an HRP-conjugated primary, ab192081 at a working dilution of 1 in 100 overnight at +4°C. The section was counterstained with haematoxylin and mounted with DPX.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre