Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17350] to Cytokeratin 14 - Cytoskeleton Marker
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker
    See all Cytokeratin 14 primary antibodies

  • Description

    Rabbit monoclonal [EPR17350] to Cytokeratin 14 - Cytoskeleton Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Rat
    ICC/IF
    Rat
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A431 and HaCaT whole cell lysates; Mouse, rat and human skin lysates. IHC-P: Human skin and squamous cell carcinoma of cervix tissues; Mouse and rat skin tissues. ICC/IF: PC-12 cells. Flow Cyt: PC-12 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17350

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Immunohistochemical analysis of paraffin-embedded human skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Immunofluorescent analysis of 4% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized PC-12 (Rat adrenal gland pheochromocytoma cells) labeling Cytokeratin 14 with ab181595 at 1/1000 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution (green). Cytoplasm staining on PC-12 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls:-
    -ve control 1: ab181595 at 1/1000 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.

  • Western blot - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Western blot - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    All lanes : Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595) at 1/20000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 2 : HaCaT (Human keratinocyte cells) whole cell lysate
    Lane 3 : Mouse skin lysate
    Lane 4 : Rat skin lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    HaCaT cells express a higher level of Cytokeratin 14 than other cell lines such as A431.

  • Flow Cytometry - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Flow Cytometry - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Flow Cytometry analysis of PC-12 (rat adrenal gland pheochromocytoma) cells labeling Cytokeratin 14 with purified ab181595 at 1/190 dilution (10 µg/mL) (red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Squamous carcinoma cells show strong staining. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

    Immunohistochemical analysis of paraffin-embedded rat skin tissue labeling Cytokeratin 14 with ab181595 at 1/2000 dilution, followed by prediluted HRP polymer for Rabbit/Mouse IgG. Basal cells of epidermis show strong staining while no staining on the stratum corneum. Counterstained with hematoxylin.

    Negative control: PBS instead of primary antibody, secondary antibody is prediluted HRP polymer for Rabbit/Mouse IgG.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Western blot - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595) at 1/20000 dilution + Human skin lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)
    Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (ab181595)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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