Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1624Y] to Cytokeratin 17 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free
    See all Cytokeratin 17 primary antibodies

  • Description

    Rabbit monoclonal [EPR1624Y] to Cytokeratin 17 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 78.6% homology with KRT36 and KRT24. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested applications

    Suitable for: WB, Flow Cyt, IP, ICC/IF, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cervical cancer tissue.

  • General notes

    ab238953 is the carrier-free version of ab51056 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab238953 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

    Unpurified ab51056 (1/50) staining human Cytokeratin 17 in paraffin embedded human squamous cervical carcinoma tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Immunoprecipitation - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunoprecipitation - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

    ab51056 (purified) at 1:20 dilution (1µg) immunoprecipitating Cytokeratin 17 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab51056 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51056 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Flow Cytometry - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Flow Cytometry - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:250 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

    Immunofluorescent staining of A431 cells using unpurified ab51056 (1/50).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • OI-RD Scanning - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    OI-RD Scanning - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue sections labeling Cytokeratin 17 with Purified ab51056 at 1:400 dilution (0.22 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51056).

  • Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)
    Anti-Cytokeratin 17 antibody [EPR1624Y] - BSA and Azide free (ab238953)

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