Antibodies, Proteins, Kits and Reagents for Life Science

Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP95] to Cytokeratin 17 - BSA and Azide free
Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
Reacts with: Human

Product name

Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free
See all Cytokeratin 17 primary antibodies
Description

Rabbit monoclonal [SP95] to Cytokeratin 17 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Human prostate carcinoma tissue ICC/IF: HeLa cells Flow Cyt (intra): HeLa cells
General notes
ab238808 is the carrier-free version of ab183330.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C. Do Not Freeze.
Storage buffer

pH: 7.20
Constituent: PBS
Carrier free

Yes
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP95
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with purified ab183330 at 1/100 (1.6µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) labeling Cytokeratin 17 with purified ab183330 at 1/20 dilution (7.85 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotypecontrol - Rabbit monoclonal IgG (ab172730) (black). Unlableled control - Unlabelled cells (blue).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Flow cytometric analysis of Cytokeratin HeLa cells using ab183330 (green) compare to negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human cervical adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human cervix adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling Cytokeratin 17 with ab183330 at 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab183330).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 17 with ab183330 at a 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab183330).
Anti-Cytokeratin 17 antibody [SP95] - BSA and Azide free (ab238808)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com