Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP95] to Cytokeratin 17 - C-terminal
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 17 antibody [SP95] - C-terminal
    See all Cytokeratin 17 primary antibodies

  • Description

    Rabbit monoclonal [SP95] to Cytokeratin 17 - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human prostate carcinoma, Human cervix. Human tonsil, Human thymus, Human cervix adenocarcinoma, Human cervical adenocarcinoma, Human bladder, Human bladder transitional cell carcinoma, Human prostate adenocarcinoma, Human prostate, Human skin and Human skin squamous cell carcinoma. ICC/IF: HeLa Flow Cyt: HeLaWB: HeLa cell lysate

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...

  • Purity

    Protein A/G purified

  • Purification notes

    Purified from TCS by protein A/G.

  • Clonality

    Monoclonal

  • Clone number

    SP95

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human skin squamous cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with purified ab183330 at 1/100 (1.6µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Flow cytometry analysis of Hela (human cervix adenocarcinoma) labeling Cytokeratin 17 with purified ab183330 at 1/20 dilution (7.85 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as a secondary antibody. Isotype control - Rabbit monoclonal IgG (ab172730) (Black). Unlableled control - Unlabelled cells (blue).

  • Western blot - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Western blot - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330) at 1/25 dilution + HeLa cell lysate

    Predicted band size: 48 kDa

  • Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Flow Cytometry - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Flow cytometric analysis of Cytokeratin HeLa cells using ab183330 (green) compare to negative control of rabbit IgG (blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human prostate tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human prostate adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human bladder transitional cell carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human cervical adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human cervix adenocarcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human cervix tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

    Immunohistochemical analysis of paraffin embedded Human prostate carcinoma tissue labeling Cytokeratin 17 with ab183330 at 1/100.

  • Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)
    Anti-Cytokeratin 17 antibody [SP95] - C-terminal (ab183330)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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