Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1623] to Cytokeratin 17 - Cytoskeleton Marker
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker
    See all Cytokeratin 17 primary antibodies

  • Description

    Rabbit monoclonal [EP1623] to Cytokeratin 17 - Cytoskeleton Marker

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431 and HACAT cell lysates Human squamous cervical carcinoma tissue Human tonsil tissue A431 cells HeLa (human cervix adenocarcinoma) cell lysate

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP1623

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Western blot - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    All lanes : Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725) at 1/1000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HACAT cell lysates

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 48 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) cells labelling Cytokeratin 17 with purified ab109725 at 1/500. Cells were fixed with 100% methanol. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

    Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

  • Flow Cytometry - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Flow Cytometry - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Flow Cytometry analysis of A431(human epidermoid carcinoma) cells labeling Cytokeratin 17 with purified ab109725 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunohistochemical analysis of paraffin-embedded human squamous cervical carcinoma tissue using ab109725 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab109725 at 1/100 dilution

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Immunofluorescent staining of A431 cells using ab109725 at 1/100 dilution
  • Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)
    Anti-Cytokeratin 17 antibody [EP1623] - Cytoskeleton Marker (ab109725)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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