Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1624Y] to Cytokeratin 17
  • Suitable for: Flow Cyt, ICC/IF, WB, IP, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Cytokeratin 17 antibody [EPR1624Y]
    See all Cytokeratin 17 primary antibodies

  • Description

    Rabbit monoclonal [EPR1624Y] to Cytokeratin 17

  • Host species

    Rabbit

  • Specificity

    The immunogen used for this product shares 78.6% homology with KRT36 and KRT24. Cross-reactivity with these proteins has not been confirmed experimentally.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cytokeratin 17 aa 400-500 (C terminal). The exact sequence is proprietary.

  • Positive control

    • WB: HeLa cell lysate IHC-P: Human cervical carcinoma tissue and Human squamous cervical carcinoma tissue ICC/IF: A431 cells. Flow Cyt: HeLa cells IP: HeLa cell lysates

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.

  • Dissociation constant (KD)

    KD = 2.47 x 10 -10 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR1624Y

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Western blot - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056) at 1/10000 dilution (Unpurified) + HeLa cell lysate at 10 µg

    Secondary
    Goat anti-Rabbit HRP labeled at 1/2000 dilution

    Predicted band size: 48 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunocytochemistry/ Immunofluorescence analysis of A431 (human epidermoid carcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:250 dilution (0.4 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunoprecipitation - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunoprecipitation - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)

    ab51056 (purified) at 1:20 dilution (1µg) immunoprecipitating Cytokeratin 17 in HeLa whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µg
    Lane 2 (+): ab51056 & HeLa whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51056 in HeLa whole cell lysate
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • Flow Cytometry - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Flow Cytometry - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cytokeratin 17 with Purified ab51056 at 1:20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cervical cancer tissue sections labeling Cytokeratin 17 with Purified ab51056 at 1:400 dilution (0.22 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylin was used as a counterstain.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)

    Unpurified ab51056 (1/50) staining human Cytokeratin 17 in paraffin embedded human squamous cervical carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)

    Immunofluorescent staining of A431 cells using unpurified ab51056 (1/50).

  • OI-RD Scanning - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    OI-RD Scanning - Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)
    Anti-Cytokeratin 17 antibody [EPR1624Y] (ab51056)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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