Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)
![Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)](https://www.abcam.com/ps/products/249/ab249329/Images/ab249329-408089-anti-inppl1-ship-2-antibody-epr10954-bsa-and-azide-free-western-blot.jpg "Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10954] to INPPL1/SHIP-2 - BSA and Azide free
- Suitable for: WB, Flow Cyt, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free
See all INPPL1/SHIP-2 primary antibodies -
Description
Rabbit monoclonal [EPR10954] to INPPL1/SHIP-2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cyt, IPmore details
Unsuitable for: ICC or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
General notes
Ab249329 is the carrier-free version of ab157460. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab249329 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR10954 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)
This data was developed using ab157460, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: INPPL1/SHIP-2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: K562 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab157460 observed at 139 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab157460 was shown to specifically react with INPPL1/SHIP-2 in wild-type HAP1 cells. No bands were observed when INPPL1/SHIP-2 knockout samples were examined. Wild-type and INPPL1/SHIP-2 knockout samples were subjected to SDS-PAGE. ab157460 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
-
![Flow Cytometry - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)](https://www.abcam.com/ps/products/249/ab249329/Images/ab249329-3-anti-inppl1-ship-2-antibody-epr10954-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329) -
![Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)](https://www.abcam.com/ps/products/249/ab249329/Images/ab249329-402600-anti-inppl1-ship-2-antibody-epr10954-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)All lanes : Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460) at 1/1000 dilution
Lane 1 : Daudi cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 139 kDaThis data was developed using ab157460, the same antibody clone in a different buffer formulation.
-
![Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)](https://www.abcam.com/ps/products/249/ab249329/Images/ab249329-399690-anti-inppl1-ship-2-antibody-epr10954-bsa-and-azide-free-immunoprecipitation.jpg)
Immunoprecipitation - Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)This data was developed using ab157460, the same antibody clone in a different buffer formulation.
Detection of INPPL1/SHIP-2 by Western Blot of Immunprecipitate. HeLa cell lysate immunoprecipitated using ab157460 at 1/10 dilution.
Anti-INPPL1/SHIP-2 antibody [EPR10954] (ab157460) at 1/1000 dilution + immunoprecipitation pellet from HeLa cell lysate
-
![Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)](https://www.abcam.com/ps/products/249/ab249329/Images/ab249329-5-benefits-of-recombinant-antibodies.png)
Anti-INPPL1/SHIP-2 antibody [EPR10954] - BSA and Azide free (ab249329)