Mouse Axl ELISA Kit (ab207619)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 10.8 pg/ml
- Range: 62.5 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Axl ELISA Kit
See all Axl kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 5 2.4% Inter-assay Sample n Mean SD CV% Serum 3 3.4% -
Sample type
Cell culture supernatant, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
10.8 pg/ml -
Range
62.5 pg/ml - 4000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 106 101% - 114% Urine 109 104% - 114% Serum 104 91% - 116% Hep Plasma 83 74% - 91% EDTA Plasma 100 86% - 111% Cit plasma 102 90% - 111% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse
Does not react with: Cow, Pig -
Product overview
Mouse Axl ELISA Kit (ab207619) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Axl protein in cell culture supernatant, serum, urine, cit plasma, edta plasma, and hep plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Axl with 10.8 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Axl is a transmembrane receptor tyrosine kinase on the plasma membrane. The growth factor GAS6 is a ligand for Axl and regulates many physiological processes including cell survival, cell proliferation, migration and differentiation. The antibodies used in this kit were raised against a portion of the extracellular domain of Axl.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Axl Capture Antibody 1 x 600µl 10X Mouse Axl Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 5BR 1 x 6ml Mouse Axl Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Relevance
The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors such as vitamin K dependent protein growth arrest specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants (AXL1 and AXL2). -
Alternative names
- Adhesion related kinase
- AI323647
- Ark
see all -
Database links
- SwissProt: Q00993 Mouse
Images
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Other - Mouse Axl ELISA Kit (ab207619)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of mouse Axl standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native Axl in mouse serum, plasma, and urine samples.The concentrations of Axl were measured in duplicates, interpolated from the Axl standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 4%, plasma (citrate) 4%, plasma (EDTA) 4%, and urine 1%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Axl concentration was determined to be 113 ng/mL in serum, 73.3 ng/mL in plasma (citrate), 88.9 ng/mL in plasma (heparin), and 112 ng/mL in urine.
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Comparison of secreted Axl in unstimulated and PMA/PHA-stimulated L929 Cells.L929 cells were grown in the absence (unstimulated) or presence of Phorbol Myristate Acetate (PMA) and phytohemagglutinin (PHA) (stimulated) for 3 days. Axl was measured in 2-fold diluted cell culture supernatants of unstimulated and PMA/PHA stimulated L929 and cell culture media. Measured values were interpolated from the Axl Standard Curve diluted in Sample Diluent NS and corrected for dilution factor. Mean of duplicate values +/-SD are graphed: 169 pg/mL unstimulated, 696 pg/mL stimulated, and undetectable in media.
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Sandwich ELISA - Mouse Axl ELISA Kit (ab207619)To learn more about the advantages of recombinant antibodies see here.
