Anti-NOX1 antibody (ab121009)
Key features and details
- Goat polyclonal to NOX1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NOX1 antibody
See all NOX1 primary antibodies -
Description
Goat polyclonal to NOX1 -
Host species
Goat -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Guinea pig, Dog, Pig, Rhesus monkey, Gorilla
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Immunogen
Synthetic peptide:
DKATDIVTGLKQK
with a Cysteine residue linker, corresponding to internal sequence amino acids 490-502 of Human NOX1 (NP_008983.2) or 441-453 of Human NOX1 (NP_039249.1). -
Positive control
- WB: HeLa and colorectal adenocarcinoma cell lysates; IHC-P: Human kidney tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab121009 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NOX1 antibody (ab121009)All lanes : Anti-NOX1 antibody (ab121009) at 0.1 µg/ml
Lane 1 : Colorectal adenocarcinoma cell lysate (35µg protein in RIPA buffer)
Lane 2 : HeLa cell lysate (35µg protein in RIPA buffer)
Predicted band size: 65 kDaPrimary incubation was 1 hour. Detected by chemiluminescence.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX1 antibody (ab121009)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling NOX1 with ab121009 at 7.5 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer pH 6. An HRP-conjugated secondary antibody was used. Counterstained with hematoxylin.
Inset: Negative control showing staining of paraffin embedded human kidney, with no primary antibody.
