Anti-CD226 (phospho S329) antibody (ab61790)
Key features and details
- Rabbit polyclonal to CD226 (phospho S329)
- Suitable for: WB, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-CD226 (phospho S329) antibody
See all CD226 primary antibodies -
Description
Rabbit polyclonal to CD226 (phospho S329) -
Host species
Rabbit -
Specificity
ab61790 detects CD226 only when phosphorylated at serine 329. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB African green monkey -
Immunogen
Synthesized phosphopeptide derived from human CD226 around the phosphorylation site of serine 329 (T-F-SP-R-R).
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Positive control
- Human brain tissue; extracts from COS7 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab61790 at 1/50-1/100 dilution with (+) or without (-) immunising phosphopeptide.
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All lanes : Anti-CD226 (phospho S329) antibody (ab61790) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells with the immunising phosphopeptide
Predicted band size: 39 kDa
Observed band size: ~ 59 kDa why is the actual band size different from the predicted?