Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1150Y] to CD8 alpha - Low endotoxin, Azide free
  • Suitable for: ICC, WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free
    See all CD8 alpha primary antibodies

  • Description

    Rabbit monoclonal [EP1150Y] to CD8 alpha - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 cell lysate. IHC-P: Human tonsil tissue. ICC: MOLT-4 cells

  • General notes

    ab210067 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CD8 alpha with purified ab93278 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93278).

  • Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    Immunocytochemistry - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    This data was developed using the same antibody clone in a different buffer formulation (ab93278).
    Immunocytochemistry analysis of MOLT-4 (human lymphoblastic leukemia T lymphoblast) labeling CD8 alpha with purified ab93278 at 1/250 dilution (9 µg/ml). Cells were fixed with 100% methanol. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.21 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
    Negative control: PBS instead of the primary antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil labelling CD8 alpha with unpurified ab93278 at a dilution of 1/250.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab93278).

  • Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)
    Anti-CD8 alpha antibody [EP1150Y] - Low endotoxin, Azide free (ab210067)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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