Anti-MHC Qa1b antibody [6A8.6F10.1A6] (ab72487)
Key features and details
- Mouse monoclonal [6A8.6F10.1A6] to MHC Qa1b
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-MHC Qa1b antibody [6A8.6F10.1A6] -
Description
Mouse monoclonal [6A8.6F10.1A6] to MHC Qa1b -
Host species
Mouse -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Mouse MHC Qa1b.
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Positive control
- ConA-stimulated Balb/c mouse splenocytes.
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General notes
ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, PBS -
Concentration information loading...
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Purity
Protein G purified -
Primary antibody notes
ab72487 can be used for flow cytometric assay to detect Qa1b on immuno-regulatory antigen presenting cells and activated splenic T cells on Qa1b expressing mice (e.g. BALB/c, C57BL/6) and it can also be used for functional assays to block target cell recognition by CTLs specific for Qa1 restricted antigen presentation and other functional study where Qa1b associated immuno-regulation may work. -
Clonality
Monoclonal -
Clone number
6A8.6F10.1A6 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow cytometric analysis of MHC Qa1b in unstimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in only a small percentage of cells.
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Flow cytometric analysis of MHC Qa1b in 72 hour ConA-stimulated Balb/c mouse splenocytes using A) isotype control and B) ab72487 (with an anti-mouse IgG1-PE secondary) at 0.25 ug/106 cells. A shift is seen in the entire population of cells.