Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATG12 antibody [EPR4800] (ab109491)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4800] to ATG12
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-ATG12 antibody [EPR4800]
    See all ATG12 primary antibodies

  • Description

    Rabbit monoclonal [EPR4800] to ATG12

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal kidney, HepG2, and Raji lysates; ATG12 transfected 293T lysates; Human breast carcinoma tissue.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)
    Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)

    Lane 1: Wild type HAP1 whole cell lysate (20 µg)
    Lane 2: ATG12 knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HeLa whole cell lysate (20 µg)
    Lane 4: HCT116 whole cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab109491 observed at 55 kDa. Observed band shows ATG12-ATG5 conjugation. Red - loading control, ab8245, observed at 37 kDa.

    Ab109491 was shown to specifically react with

    ATG12 in wild-type cells along with additional cross-reactive bands. The band was not seen in ATG12 knockout HAP1 cells. Wild-type and ATG12 knockout samples were subjected to SDS-PAGE.  Ab109491 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)
    Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)
    All lanes : Anti-ATG12 antibody [EPR4800] (ab109491) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : HepG2 lysate
    Lane 3 : Raji lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa



    Observed band shows ATG12-ATG5 conjugation

  • Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)
    Western blot - Anti-ATG12 antibody [EPR4800] (ab109491)
    All lanes : Anti-ATG12 antibody [EPR4800] (ab109491) at 1/1000 dilution

    Lane 1 : ATG12 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 15 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody [EPR4800] (ab109491)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody [EPR4800] (ab109491)

    ab109491, at 1/250 dilution, staining ATG12 in paraffin-embedded Human breast carcinoma by Immunohistochemistry.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-ATG12 antibody [EPR4800] (ab109491)
    Anti-ATG12 antibody [EPR4800] (ab109491)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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