Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-UFSP2 antibody [EP13424-49] (ab192597)

Key features and details

  • Rabbit monoclonal [EP13424-49] to UFSP2
  • Suitable for: WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-UFSP2 antibody [EP13424-49]
    See all UFSP2 primary antibodies

  • Description

    Rabbit monoclonal [EP13424-49] to UFSP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal kidney, fetal heart and mouse spleen lysates; HEK293T, HeLa, rat C6, mouse Raw 264.7 and mouse NIH 3T3 cell lysates. IHC-P: Human thyroid carcinoma tissue. ICC/IF: HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EP13424-49

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : UFSP2 knockout HEK293T cell lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 53 kDa
    Observed band size: 53 kDa



    Lanes 1-3: Merged signal (red and green). Green - ab192597 observed at 53 kDa. Red - loading control ab8245 observed at 36 kDa.

     ab192597 Anti-UFSP2 antibody [EP13424-49] was shown to specifically react with UFSP2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266368 (knockout cell lysate ab257782) was used. Wild-type and UFSP2 knockout samples were subjected to SDS-PAGE. ab192597 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-UFSP2 antibody [EP13424-49] (ab192597)

    Immunohistochemical analysis of paraffin-embedded, Human thyroid carcinoma tissue labeling UFSP2 with ab192597 at a 1/100 dilution. Counter stained with hematoxylin. Negative control also shown.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    Immunocytochemistry/ Immunofluorescence - Anti-UFSP2 antibody [EP13424-49] (ab192597)

    Immunofluorescence analysis of, paraformaldehyde-fixed, HeLa cells labeling UFSP2 with ab192597 at a 1/100 dilution. A secondary antibody goat anti-rabbit IgG (Alexa Fluor®488) ab150077 was used at a 1/400 dilution. In blue DAPI staining.

  • Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/10000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate
    Lane 3 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

  • Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    Western blot - Anti-UFSP2 antibody [EP13424-49] (ab192597)
    All lanes : Anti-UFSP2 antibody [EP13424-49] (ab192597) at 1/1000 dilution

    Lane 1 : Mouse spleen lysate
    Lane 2 : rat C6 cell lysate
    Lane 3 : mouse Raw 264.7 cell lysate
    Lane 4 : mouse NIH 3T3 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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