Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-APLP2 antibody [EPR5938(2)] (ab140624)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5938(2)] to APLP2
  • Suitable for: IHC-P, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-APLP2 antibody [EPR5938(2)]
    See all APLP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR5938(2)] to APLP2

  • Host species

    Rabbit

  • Specificity

    ab140624 detects isoforms in some cell lines.

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human APLP2 aa 200-300. The exact sequence is proprietary.
    Database link: Q06481

  • Positive control

    • Fetal kidney, HeLa, placenta, SH-SY5Y, fetal brain and 293T lysates; Human kidney tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    Western blot - Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/50000 dilution + LNCaP(Human prostate carcinoma epithelial cell)whole cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa
    Observed band size: 90-120 kDa
    why is the actual band size different from the predicted?


    Exposure time: 30 seconds


    Diluting/Blocking buffer: 5% NFDM /TBST

    ab140624 detects isoforms in some cell lines and the bands are consistent with the literature (PMID: 16279945). 

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-APLP2 antibody [EPR5938(2)] (ab140624)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling APLP2 with ab140624 at 1/100 dilution.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    Western blot - Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    All lanes : Anti-APLP2 antibody [EPR5938(2)] (ab140624) at 1/10000 dilution

    Lane 1 : Fetal kidney tissue lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Placenta tissue lysate
    Lane 4 : SH-SY5Y cell lysate
    Lane 5 : Fetal brain tissue lysate
    Lane 6 : 293T cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 87 kDa

  • Anti-APLP2 antibody [EPR5938(2)] (ab140624)
    Anti-APLP2 antibody [EPR5938(2)] (ab140624)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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