Anti-ATG12 antibody (ab240900)
Key features and details
- Rabbit polyclonal to ATG12
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG12 antibody
See all ATG12 primary antibodies -
Description
Rabbit polyclonal to ATG12 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ATG12 (N terminal). (NP_001264712.1).
Database link: O94817 -
Positive control
- WB: HeLa cell lysate. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody (ab240900)
Immunofluorescent analysis of 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATG12 with ab240900 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Rabbit ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue), used at a 1/5000 dilution for 5 minutes at RT.
A: DAPI
B: ATG12
C: Composite.
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Western blot - Anti-ATG12 antibody (ab240900)Anti-ATG12 antibody (ab240900) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit: HRP at 1/2000 dilution
Exposure time: 12 minutesPrimary incubation for 1 hour at room temperature.
Blocking buffer: 5% milk + TBST.
