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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Ub-like Proteins

Anti-ATG12 antibody (ab155589)

Price and availability

284 784 ₸

Availability

Order now and get it on Wednesday March 03, 2021

Anti-ATG12 antibody (ab155589)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to ATG12
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-ATG12 antibody
    See all ATG12 primary antibodies
  • Description

    Rabbit polyclonal to ATG12
  • Host species

    Rabbit
  • Specificity

    ab155589 detects free ATG12 and ATG12 conjugated to Atg5.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human ATG12 aa 1-187. The exact sequence is proprietary.

  • Positive control

    • 293T, A431, NIH3T3 and Rat brain whole cell lysates; Human U87 xenograft tissue; HeLa cells untreated or treated with Tunicamycin.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Ub-like Proteins
    • Cell Biology
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Autophagy
    • APG gene products
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • APG gene products
    • Cancer
    • Cell Death
    • Apoptosis
    • Phagocytosis
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
    • Cancer
    • Cell Death
    • Autophagy
    • APG gene products

Images

  • Western blot - Anti-ATG12 antibody (ab155589)
    Western blot - Anti-ATG12 antibody (ab155589)
    Anti-ATG12 antibody (ab155589) at 1/1000 dilution + NIH3T3 whole cell lysate at 30 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Western blot - Anti-ATG12 antibody (ab155589)
    Western blot - Anti-ATG12 antibody (ab155589)
    Anti-ATG12 antibody (ab155589) at 1/1000 dilution + Rat brain whole cell lysate at 50 µg

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Western blot - Anti-ATG12 antibody (ab155589)
    Western blot - Anti-ATG12 antibody (ab155589)
    All lanes : Anti-ATG12 antibody (ab155589) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 15 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody (ab155589)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody (ab155589)

    Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling ATG12 with ab155589 at 1/500 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody (ab155589)
    Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody (ab155589)

    Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ATG12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
    Cells were fixed in 4% paraformaldehyde for 15 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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