Recombinant human USP9x protein (Tagged) (ab271784)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus, DDDDK tag C-Terminus
- Suitable for: Functional Studies, SDS-PAGE
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Product name
Recombinant human USP9x protein (Tagged)
See all USP9x proteins and peptides -
Biological activity
Specific Activity: 70 pmol/min/μg
Assay Conditions: 50mM Tris-HCl, 0.5mM EDTA, 0.05% Tween 20, 1mM DTT, 500nM Ubiquitin-AMC. Incubate at room temperature for 30 minutes. Fluorescence intensity is measured at exc/em of 360nm/460nm using Tecan M1000 plate reader.
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Purity
>= 90 % SDS-PAGE. -
Expression system
HEK 293 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
TATTRGSPVG GNDNQGQAPD GQSQPPLQQN QTSSPDSSNE NSPATPPDEQ GQGDAPPQLE DEEPAFPHTD LAKLDDMINR PRWVVPVLPK GELEVLLEAA IDLSKKGLDV KSEACQRFFR DGLTISFTKI LTDEAVSGWK FEIHRCIINN THRLVELCVA KLSQDWFPLL ELLAMALNPH CKFHIYNGTR PCESVSSSVQ LPEDELFARS PDPRSPKGWL VDLLNKFGTL NGFQILHDRF INGSALNVQI IAALIKPFGQ CYEFLTLHTV KKYFLPIIEM VPQFLENLTD EELKKEAKNE AKNDALSMII KSLKNLASRV PGQEETVKNL EIFRLKMILR LLQISSFNGK MNALNEVNKV ISSVSYYTHR HGNPEEEEWL TAERMAEWIQ QNNILSIVLR DSLHQPQYVE KLEKILRFVI KEKALTLQDL DNIWAAQAGK HEAIVKNVHD LLAKLAWDFS PEQLDHLFDC FKASWTNASK KQREKLLELI RRLAEDDKDG VMAHKVLNLL WNLAHSDDVP VDIMDLALSA HIKILDYSCS QDRDTQKIQW IDRFIEELRT NDKWVIPALK QIREICSLFG EAPQNLSQTQ RSPHVFYRHD LINQLQHNHA LVTLVAENLA TYMESMRLYA RDHEDYDPQT VRLGSRYSHV QEVQERLNFL RFLLKDGQLW LCAPQAKQIW KCLAENAVYL CDREACFKWY SKLMGDEPDL DPDINKDFFE SNVLQLDPSL LTENGMKCFE RFFKAVNCRE GKLVAKRRAY MMDDLELIGL DYLWRVVIQS NDDIASRAID LLKEIYTNLG PRLQVNQVVI HEDFIQSCFD RLKASYDTLC VLDGDKDSVN CARQEAVRMV RVLTVLREYI NECDSDYHEE RTILPMSRAF RGKHLSFVVR FPNQGRQVDD LEVWSHTNDT IGSVRRCILN RIKANVAHTK IELFVGGELI DPADDRKLIG QLNLKDKSLI TAKLTQISSN MPSSPDSSSD SSTGSPGNHG NHYSDGPNPE VESCLPGVIM SLHPRYISFL WQVADLGSSL NMPPLRDGAR VLMKLMPPDS TTIEKLRAIC LDHAKLGESS LSPSLDSLFF GPSASQVLYL TEVVYALLMP AGAPLADDSS DFQFHFLKSG GLPLVLSMLT RNNFLPNADM ETRRGAYLNA LKIAKLLLTA IGYGHVRAVA EACQPGVEGV NPMTQINQVT HDQAVVLQSA LQSIPNPSSE CMLRNVSVRL AQQISDEASR YMPDICVIRA IQKIIWASGC GSLQLVFSPN EEITKIYEKT NAGNEPDLED EQVCCEALEV MTLCFALIPT ALDALSKEKA WQTFIIDLLL HCHSKTVRQV AQEQFFLMCT RCCMGHRPLL FFITLLFTVL GSTARERAKH SGDYFTLLRH LLNYAYNSNI NVPNAEVLLN NEIDWLKRIR DDVKRTGETG IEETILEGHL GVTKELLAFQ TSEKKFHIGC EKGGANLIKE LIDDFIFPAS NVYLQYMRNG ELPAEQAIPV CGSPPTINAG FELLVALAVG CVRNLKQIVD SLTEMYYIGT AITTCEALTE WEYLPPVGPR PPKGFVGLKN AGATCYMNSV IQQLYMIPSI RNGILAIEGT GSDVDDDMSG DEKQDNESNV DPRDDVFGYP QQFEDKPALS KTEDRKEYNI GVLRHLQVIF GHLAASRLQY YVPRGFWKQF RLWGEPVNLR EQHDALEFFN SLVDSLDEAL KALGHPAMLS KVLGGSFADQ KICQGCPHRY ECEESFTTLN VDIRNHQNLL DSLEQYVKGD LLEGANAYHC EKCNKKVDTV KRLLIKKLPP VLAIQLKRFD YDWERECAIK FNDYFEFPRE LDMEPYTVAG VAKLEGDNVN PESQLIQQSE QSESETAGST KYRLVGVLVH SGQASGGHYY SYIIQRNGGD GERNRWYKFD DGDVTECKMD DDEEMKNQCF GGEYMGEVFD HMMKRMSYRR QKRWWNAYIL FYERMDTIDQ DDELIRYISE LAITTRPHQI IMPSAIERSV RKQNVQFMHN RMQYSMEYFQ FMKKLLTCNG VYLNPPPGQD HLLPEAEEIT MISIQLAARF LFTTGFHTKK VVRGSASDWY DALCILLRHS KNVRFWFAHN VLFNVSNRFS EYLLECPSAE VRGAFAKLIV FIAHFSLQDG PCPSPFASPG PSSQAYDNLS LSDHLLRAVL NLLRREVSEH GRHLQQYFNL FVMYANLGVA EKTQLLKLSV PATFMLVSLD EGPGPPIKYQ YAELGKLYSV VSQLIRCCNV SSRMQSSING NPPLPNPFGD PNLSQPIMPI QQNVADILFV RTSYVKKIIE DCSNSEETVK LLRFCCWENP QFSSTVLSEL LWQVAYSYTY ELRPYLDLLL QILLIEDSWQ THRIHNALKG IPDDRDGLFD TIQRSKNHYQ KRAYQCIKCM VALFSNCPVA YQILQGNGDL KRKWTWAVEW LGDELERRPY TGNPQYTYNN WSPPVQSNET SNGYFLERSH SARMTLAKAC ELCPEEEPDD QDAPDEHESP PPEDAPLYPH SPGSQYQQNN HVHGQPYTGP AAHHMNNPQR TGQRAQENYE GSEEVSPPQT KDQ -
Predicted molecular weight
293 kDa including tags -
Amino acids
2 to 2554 -
Tags
His tag N-Terminus , DDDDK tag C-Terminus
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Preparation and Storage
- Deubiquitinating enzyme FAF X
- Deubiquitinating enzyme FAF-X
- DFFRX
Images
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SDS-PAGE - Recombinant Human USP9x protein (Tagged) (ab271784)
SDS-PAGE analysis of ab271784.
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Functional Studies - Recombinant Human USP9x protein (Tagged) (ab271784)Functional studies of ab271784 was 70 pmol/min/μg.
