Anti-ATG10 antibody (ab240901)
Key features and details
- Rabbit polyclonal to ATG10
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ATG10 antibody
See all ATG10 primary antibodies -
Description
Rabbit polyclonal to ATG10 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Synthetic peptide corresponding to Human ATG10 (internal sequence). (NP_001124500.1).
Database link: Q9H0Y0 -
Positive control
- WB: HEK-293T cell lysate. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ATG10 antibody (ab240901)
Immunofluorescent analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ATG10 with ab240901 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Rabbit ATTO 488 at 1/100 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).
A: DAPI, B: Actin, C: ATG10, D: Composite.
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Western blot - Anti-ATG10 antibody (ab240901)Anti-ATG10 antibody (ab240901) at 1/1000 dilution + HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG: HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 25 kDa
Exposure time: 6 minutesPrimary incubation for 1 hour at room temperature.
Block: 5% Skim Milk in 1X TBST
