Anti-RPL27A antibody (ab74731)
Key features and details
- Rabbit polyclonal to RPL27A
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RPL27A antibody
See all RPL27A primary antibodies -
Description
Rabbit polyclonal to RPL27A -
Host species
Rabbit -
Specificity
ab74731 detects endogenous levels of total RPL27A protein. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
synthesized peptide derived from C-terminal region of human RPL27A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RPL27A antibody (ab74731)All lanes : Anti-RPL27A antibody (ab74731) at 1/500 dilution
Lane 1 : extracts from COLO cells
Lane 2 : extracts from COLO cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 17 kDa
Observed band size: 19 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands.
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Immunocytochemistry/ Immunofluorescence - Anti-RPL27A antibody (ab74731)Immunofluorescence analysis of A549 cells, using ab74731 at 1/500 dilution with (+) or without (-) immunising peptide.
