Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22168] to RPS6 - BSA and Azide free
  • Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-RPS6 antibody [EPR22168] - BSA and Azide free
    See all RPS6 primary antibodies

  • Description

    Rabbit monoclonal [EPR22168] to RPS6 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cerebral cortex tissue.

  • General notes

    ab236454 is the carrier-free version of ab225676.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR22168

  • Isotype

    IgG

  • Research areas

Images

  • Flow Cytometry - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Flow Cytometry - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RPS6 with ab225676 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the rat cerebellum (PMID: 26432888). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the mouse cerebrum (PMID: 26432888). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining in human breast cancer (PMID: 22698676). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (Mouse embryo fibroblast cell line) cells labeling RPS6 with ab225676 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

  • Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunocytochemistry/ Immunofluorescence - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling RPS6 with ab225676 at 1/500 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in the HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

    Immunohistochemical analysis of paraffin-embedded human cerebral cortex tissue labeling RPS6 with ab225676 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Cytoplasmic staining predominantly in neurons of the human cerebral cortex (PMID: 26432888). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab225676).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)
    Anti-RPS6 antibody [EPR22168] - BSA and Azide free (ab236454)

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