Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RPL9 antibody [EP13752] (ab182556)

Key features and details

  • Rabbit monoclonal [EP13752] to RPL9
  • Suitable for: IP, WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-RPL9 antibody [EP13752]
    See all RPL9 primary antibodies

  • Description

    Rabbit monoclonal [EP13752] to RPL9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, HeLa, HTC-116 and Jurkat whole cell lysate (ab7899); Human brain tissue; HTC-116 and HeLa cells.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EP13752

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-RPL9 antibody [EP13752] (ab182556)
    Western blot - Anti-RPL9 antibody [EP13752] (ab182556)
    All lanes : Anti-RPL9 antibody [EP13752] (ab182556) at 1/10000 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : HCT-116 cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 22 kDa
    Observed band size: 22 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody [EP13752] (ab182556)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPL9 antibody [EP13752] (ab182556)

    Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling RPL9 with ab182556 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody [EP13752] (ab182556)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody [EP13752] (ab182556)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody [EP13752] (ab182556)
    Immunocytochemistry/ Immunofluorescence - Anti-RPL9 antibody [EP13752] (ab182556)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling RPL9 with ab182556 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunoprecipitation - Anti-RPL9 antibody [EP13752] (ab182556)
    Immunoprecipitation - Anti-RPL9 antibody [EP13752] (ab182556)

    Western blot analysis of HepG2 cell lysate immunoprecipitated with ab182556 at 1/50 dilution (Lane 1). Lane 2: Negative control.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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