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Cell Biology Proteolysis / Ubiquitin Proteasome / Ubiquitin Proteasome

Anti-NUB1 antibody [EPR10717] (ab178414)

Price and availability

298 185 ₸

Availability

Order now and get it on Wednesday March 17, 2021

Anti-NUB1 antibody [EPR10717] (ab178414)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10717] to NUB1
  • Suitable for: Flow Cyt, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-NUB1 antibody [EPR10717]
    See all NUB1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10717] to NUB1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human NUB1 aa 400-500. The exact sequence is proprietary.
    Database link: Q9Y5A7

  • Positive control

    • Raji, 293T, HeLa and Jurkat cell lysates; Jurkat cells.
  • General notes

     This product was previously labelled as NYREN18, Nubbin

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10717
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Proteasome

Images

  • Flow Cytometry - Anti-NUB1 antibody [EPR10717] (ab178414)
    Flow Cytometry - Anti-NUB1 antibody [EPR10717] (ab178414)

    Flow Cytometrical analysis of permeabilized Jurkat cells labeling NYREN18 with ab178414 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-NUB1 antibody [EPR10717] (ab178414)
    Western blot - Anti-NUB1 antibody [EPR10717] (ab178414)
    All lanes : Anti-NUB1 antibody [EPR10717] (ab178414) at 1/1000 dilution

    Lane 1 : Raji cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Jurkat cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 71 kDa
    Additional bands at: 71 kDa. We are unsure as to the identity of these extra bands.

  • Anti-NUB1 antibody [EPR10717] (ab178414)
    Anti-NUB1 antibody [EPR10717] (ab178414)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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