Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
![Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-266921-anti-proteasome-20s-lmp2-antibody-epr13784-western-blot.jpg "Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2
- Suitable for: WB, ICC/IF, Flow Cyt, IP
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Proteasome 20S LMP2 antibody [EPR13784]
See all Proteasome 20S LMP2 primary antibodies -
Description
Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt HumanICC/IF HumanIP HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Ramos, Raji, HL60 and A431 cell lysates; A431 and Raji cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13784 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-220630-anti-proteasome-20s-lmp2-antibody-epr13784-western-blot.jpg)
Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
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![Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-220629-anti-proteasome-20s-lmp2-antibody-epr13784-western-blot.jpg)
Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution
Lane 1 : HL60 cell lysate
Lane 2 : A431 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 23 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
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![Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-220633-anti-proteasome-20s-lmp2-antibody-epr13784-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1). Lane 2: PBS instead of Raji lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.
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![Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-220632-anti-proteasome-20s-lmp2-antibody-epr13784-flow-cytometry.jpg)
Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)Flow cytometric analysis of 2% paraformaldehyde-fixed Raji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-220631-anti-proteasome-20s-lmp2-antibody-epr13784-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).
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![Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)](https://www.abcam.com/ps/products/187/ab187645/Images/ab187645-7-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
