Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2
  • Suitable for: WB, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Proteasome 20S LMP2 antibody [EPR13784]
    See all Proteasome 20S LMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR13784] to Proteasome 20S LMP2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Ramos, Raji, HL60 and A431 cell lysates; A431 and Raji cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13784

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
    Lanes 1 - 2: Merged signal (red and green). Green - ab187645 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
    ab187645 was shown to recognize Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used, along with additional cross-reactive bands. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab187645 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/10000 dilution

    Lane 1 : Ramos cell lysate
    Lane 2 : Raji cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    All lanes : Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645) at 1/2000 dilution

    Lane 1 : HL60 cell lysate
    Lane 2 : A431 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 21 kDa why is the actual band size different from the predicted?

  • Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Immunoprecipitation - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Western blot analysis of Proteasome 20S LMP2 in Raji cell lysate immunoprecipitated using ab187645 at 1/50 (Lane 1).  Lane 2: PBS instead of Raji lysate. 

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    Based on the sequence analysis by BLAST, ab187645 recognizes two isoforms with the predicted MWs of 22kDa and 23kDa respectively.

  • Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Flow Cytometry - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Flow cytometric analysis of 2% paraformaldehyde-fixed Raji cells labeling Proteasome 20S LMP2 with ab187645 at 1/170 dilution compared to a Rabbit monoclonal IgG isotype control, followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling Proteasome 20S LMP2 with ab187645 at 1/100 dilution. Counter stained with Dapi (blue).

  • Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)
    Anti-Proteasome 20S LMP2 antibody [EPR13784] (ab187645)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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