Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)
![Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)](https://www.abcam.com/ps/products/238/ab238435/Images/ab238435-332206-23843522.jpg "Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2 - BSA and Azide free
- Suitable for: WB, ICC/IF, IP
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
-
Product name
Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free
See all Proteasome 20S LMP2 primary antibodies -
Description
Rabbit monoclonal [EPR13785] to Proteasome 20S LMP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Wild-type HAP1 whole cell lysate. ICC/IF: HL-60 cells.
-
General notes
Ab238435 is the carrier-free version of ab184172. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab238435 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR13785 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Proteasome 20S LMP2 knockout HAP1 cell lysate (20 µg)
Lanes 1 - 2: Merged signal (red and green). Green - ab184172 observed at 22 and 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab184172 was shown to specifically react with Proteasome 20S LMP2 when Proteasome 20S LMP2 knockout samples were used. Wild-type and Proteasome 20S LMP2 knockout samples were subjected to SDS-PAGE. ab184172 and ab8245 (loading control to GAPDH) were both diluted 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab184172).
-
![Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)](https://www.abcam.com/ps/products/238/ab238435/Images/ab238435-332205-ab985961.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)Immunofluorescent analysis of 4% paraformaldehyde fixed HL-60 (Human promyelocytic leukemia cell line) cells labeling Proteasome 20S LMP2 using ab184172 at 1/100 dilution (red). A Goat anti rabbit IgG (Alexa Fluor®555) at 1/200 dilution was used as secondary antibody. Counterstain: DAPI (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab184172).
-
![Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)](https://www.abcam.com/ps/products/238/ab238435/Images/ab238435-3-benefits-of-recombinant-antibodies.png)
Anti-Proteasome 20S LMP2 antibody [EPR13785] - BSA and Azide free (ab238435)
