Anti-Proteasome 20S LMP2 antibody (ab3328)
Key features and details
- Rabbit polyclonal to Proteasome 20S LMP2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Proteasome 20S LMP2 antibody
See all Proteasome 20S LMP2 primary antibodies -
Description
Rabbit polyclonal to Proteasome 20S LMP2 -
Host species
Rabbit -
Specificity
Detects proteasome 20S LMP2. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
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Immunogen
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 0.1% BSA, 99% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)All lanes : Anti-Proteasome 20S LMP2 antibody (ab3328) at 2 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 100 ng/mL IFN gamma for 72 hours, whole cell lysate
Lane 3 : THP-1 (human monocytic leukemia cell line) whole cell lysate
Lane 4 : THP-1 (human monocytic leukemia cell line) treated with 50 ng/mL PMA for 48 hours and 200 ng/mL LPS for 24 hours, whole cell lysate
Lane 5 : U937 (human histiocytic lymphoma cell line) whole cell lysate
Lane 6 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 7 : Ramos (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) HRP conjugate at 0.4 µg/ml
Developed using the ECL technique.
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Western blot - Anti-Proteasome 20S LMP2 antibody (ab3328)Western blot of proteasome 20S LMP2 in 50 ug mouse embryo fibroblast cell extract using ab3328.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328)ab3328 (1µg/ml) staining Proteasome 20S LMP2 in human heart using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear and cytoplasmic cell compartments.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1/ EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Proteasome 20S LMP2 antibody (ab3328) Image from Nguyen TP et al., Am J Pathol. 2010;176(2):893-902. Fig 8(A).; doi: 10.2353/ajpath.2010.090390 with permission from Elsevier.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human brain tissue (neurons in HIV encephalitis) sections labelling LMP2 with ab3328 at 1/1000. Immunofluorescence staining for LMP2 shows colocalization with the neuronal marker NeuN. LMP2 is present in granular deposits in the parikaryon. Scale bar = 10 μm.
