Anti-GABRB1 (phospho S434) antibody (ab51123)
Key features and details
- Rabbit polyclonal to GABRB1 (phospho S434)
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human, African green monkey
- Isotype: IgG
Overview
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Product name
Anti-GABRB1 (phospho S434) antibody
See all GABRB1 primary antibodies -
Description
Rabbit polyclonal to GABRB1 (phospho S434) -
Host species
Rabbit -
Specificity
Ab51123 detects endogenous levels of GABRB1 only when phosphorylated at serine 434.
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Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, African green monkey
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide corresponding to Human GABRB1.
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Positive control
- Human breast carcinoma tissue or extracts from cos7 cells.
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General notes
This product was previously labelled as GABA A Receptor beta 1
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GABRB1 (phospho S434) antibody (ab51123)All lanes : Anti-GABRB1 (phospho S434) antibody (ab51123)
Lane 1 : Extracts from cos7 cells with immunising peptide
Lane 2 : Extracts from cos7 cells with no peptide
Predicted band size: 53 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 (phospho S434) antibody (ab51123)This image shows paraffin-embedded human breast carcinoma tissue stained with ab51123 at a dilution of 1/100. Right hand image: tissue treated with immunising peptide; left hand image: untreated tissue -
Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 (phospho S434) antibody (ab51123)ICC/IF image of ab51123 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51123, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
