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Neuroscience Neurotransmitter Transporters GABA

Anti-GABRB1 antibody [N96/55] (ab93612)

Anti-GABRB1 antibody [N96/55] (ab93612)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Mouse monoclonal [N96/55] to GABRB1
  • Suitable for: IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-GABRB1 antibody [N96/55]
    See all GABRB1 primary antibodies
  • Description

    Mouse monoclonal [N96/55] to GABRB1
  • Host species

    Mouse
  • Specificity

    No cross-reactivity against GABA A Receptor beta 2 or GABA A Receptor beta 3.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Mouse
    WB
    Rat
    See all applications and species data
  • Immunogen

    Fusion protein corresponding to Mouse GABRB1 aa 327-450 (C terminal).
    Database link: P50571

  • Positive control

    • WB: rat brain lysate. IHC-P: Mouse backskin tissue. ICC/IF: HaCat cells.
  • General notes

    The clone number has been updated from S96-55 to N96/55, both clone numbers name the same antibody clone.

     

     This product was previously labelled as GABA A Receptor beta 1

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 50% Glycerol (glycerin, glycerine), PBS
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    N96/55
  • Isotype

    IgG1
  • Research areas

    • Neuroscience
    • Neurotransmitter
    • Amino Acids
    • GABA
    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • GABA Receptors
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Types of disease
    • Cancer

Images

  • Western blot - Anti-GABRB1 antibody [N96/55] (ab93612)
    Western blot - Anti-GABRB1 antibody [N96/55] (ab93612)

    Western blot detection of GABRB1 in Rat brain membrane lysates using ab96612 at 1:1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 antibody [N96/55] (ab93612)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABRB1 antibody [N96/55] (ab93612)

    Paraffin-embedded mouse backskin tissue fixed with Bouin's fixative, stained for GABRB1 using ab93612 at 1/100 dilution in immunohistochemical analysis. Primary antibody was incubated for 1 hour at room temperature. FITC-conjugated goat anti-mouse (green) at 1/50 was used as the secondary antibody. Secondary was incubated at 1 hour at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 antibody [N96/55] (ab93612)
    Immunocytochemistry/ Immunofluorescence - Anti-GABRB1 antibody [N96/55] (ab93612)

    HaCaT (Human keratinocyte cell line) cells stained gor GABRB1 using ab93612 at 1/100 dilution in ICC/IF. Cells were fixed using 100% methanol for 10 minutes at -20°C and incubated with primary antibody for 1 hour at room temperature. Secondary antibody was FITC-conjugated goat anti-mouse (green) at 1/50 incubated for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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