Succinate Assay Kit (Colorimetric) (ab204718)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 30 min
- Sample type: Adherent cells, Suspension cells, Tissue
- Sensitivity: 40 µM
Overview
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Product name
Succinate Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Tissue, Adherent cells, Suspension cells -
Assay type
Quantitative -
Sensitivity
40 µM -
Assay time
0h 30m -
Species reactivity
Reacts with: Mammals, Other species -
Product overview
Succinate Assay Kit (Colorimetric) (ab204718) is a sensitive, fast and easy-to-use kit. In the succinate assay protocol, Succinate is utilized by Succinyl-CoA Synthetase to form an intermediate, which undergoes a series of reactions and reduces a colorless probe to a colored product with strong absorbance at OD=450 nm. This assay kit can detect less than 40 µM of succinate or succinic acid in a variety of sample types.
Succinate + ATP + CoA converted by succiny-CoA synthase to succiny-CoA + ADP + Pi with Color Detection (OD 450 nm).
Succinate assay protocol summary:
- add samples and standards to wells
- add reaction mix and incubate for 30 min
- analyze with a microplate reader -
Notes
Succinic acid (C4H6O4) is distributed in all plants and animal tissues and was first obtained from amber. Due to its low toxicity, it is widely used in agriculture, food and pharmaceutical industry. Succinate (C4H4O4) is the salt or ester of succinic acid. It is one of the most active components of cellular respiratory and intracellular energy generation. Measurement of succinate or succinic acid level is a key to analysis of the citric acid cycle.
This kit has so far only been tested with human and rat samples. Since Succinate is distributed in all plants and animal tissues this kit should detect Succinate independent of the species. Depending on the sample type, the sample preparation might need to be optimized.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Succinate Assay Buffer 1 x 25ml Succinate Converter (Lyophilized) 1 vial Succinate Developer (Lyophilized) 1 vial Succinate Enzyme Mix (Lyophilized) 1 vial Succinate Standard (Lyophilized) 1 vial Succinate Substrate Mix (Lyophilized) 1 vial -
Research areas
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Relevance
Succinic acid is involved in the catabolism of the neurotransmitter gamma-aminobutyric acid (GABA). -
Alternative names
- butanedioic acid
- ethane-12 dicarboxylic acid
- ethylenesuccinic acid
see all
Images
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Succinate Assay Kit (Colorimetric) (ab204718)
Succinate Standard Curve.
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Succinate Assay Kit (Colorimetric) (ab204718)Measurement of Succinate levels in rat liver (40 µg), kidney (40 µg) and muscle (40 µg), Jurkat cell lysate (200 µg) and white wine (4 µl, wine sample was diluted 1:1 with 0.5 M Tris HCl, pH 8.0).