Recombinant dog PDE4D protein (ab198425)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
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Product name
Recombinant dog PDE4D protein
See all PDE4B proteins and peptides -
Biological activity
Specific Activity: ≥ 5665 pmol/min/µg
Unit Definition: One unit is defined as the amount of ab198425 that will convert 1 pmole of 3, 5-cAMP to 5-AMP per minute at 37°C.
Assay Conditions: 10 mM Tris-HCl, pH 7.4, 10 mM MgCl2, 0.1 mg/ml BSA, 0.05% Tween-20, 200µM cAMP, 2.5 kU 5'-nucleotidase, and serial dilutions of ab198425 at 37°C for 20 min. Quantified by 5'- nucleotidase cleaving the 5' -AMP product and releasing the phosphate group which is detected by Malachite Green Reagent. -
Purity
> 90 % SDS-PAGE. -
Expression system
Baculovirus infected Sf9 cells -
Accession
-
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Dog -
Sequence
MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGL EFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVL DIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFKDRLCHKTYLNGDHVTH PDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIA WPLQGWQATFGGGDHLVPRGSGSGIQSIIMKPRSRSTSSLRTTEAACFDV DNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKS MSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRS PMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMAS NKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQHEVEIPSPTQKEKE KKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWG LHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHY HADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVD HPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLT KKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNY SDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGME ISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNR EWYQSTIPQSPSPAPDEQEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQ VEEDTSCSDSKTLCTQDSESTEIPLDEQVEEETVGEEENSQPEACVIEDH SPDT -
Predicted molecular weight
104 kDa including tags -
Amino acids
2 to 679 -
Tags
GST tag N-Terminus
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Description
Recombinant dog PDE4B protein
Preparation and Storage
- 5''-cyclic phosphodiesterase 4B
- cAMP-specific 3''
- cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Images
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SDS-PAGE - Recombinant dog PDE4D protein (ab198425)
4-20% SDS-PAGE analysis of ab198425 with Coomassie staining.
Lane 1: 2 µg ab198425
Lane 2: Protein Marker -
Functional Studies - Recombinant dog PDE4D protein (ab198425)Specific activity of ab198425.
