Anti-PDE6 alpha antibody (ab5659)
Key features and details
- Rabbit polyclonal to PDE6 alpha
- Suitable for: IHC-P, WB
- Reacts with: Sheep, Human
- Isotype: IgG
Overview
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Product name
Anti-PDE6 alpha antibody
See all PDE6 alpha primary antibodies -
Description
Rabbit polyclonal to PDE6 alpha -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Sheep
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Immunogen
Synthetic peptide corresponding to Human PDE6 alpha aa 1-16.
Sequence:MGEVTAEEVEKFLDSN
(Peptide available asab5862)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PDE6 alpha antibody (ab5659)
Western blot of PDE6 alpha on sheep retinal extracts using ab5659.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDE6 alpha antibody (ab5659)IHC image of ab5659 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab5659, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
