Anti-TORC1 antibody (ab264144)
Key features and details
- Rabbit polyclonal to TORC1
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-TORC1 antibody
See all TORC1 primary antibodies -
Description
Rabbit polyclonal to TORC1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide within Human TORC1 aa 600-634 (C terminal). The exact sequence is proprietary.
Database link: Q6UUV9 -
Positive control
- WB: HeLa, HEK-293T, TCMK and NIH/3T3 whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human prostate carcinoma.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TORC1 antibody (ab264144)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling TORC1 with ab264144 at 1/200 dilution. Detection: DAB.
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Western blot - Anti-TORC1 antibody (ab264144)All lanes : Anti-TORC1 antibody (ab264144) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Western blot - Anti-TORC1 antibody (ab264144)All lanes : Anti-TORC1 antibody (ab264144) at 0.1 µg/ml
Lane 1 : TCMK (Mouse kidney epithelial cell line) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 3 minutes4-8% SDS-PAGE.
Cells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-TORC1 antibody (ab264144)TORC1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264144 at 3 µg per reaction. Western blot was performed from the immunoprecipitate using ab264144 at 1 µg/ml.
Lane 1: ab264144 IP in HeLa whole cell lysate.
Lane 2: Control IgG.Exposure time: 3 min.
