Alcohol Dehydrogenase Assay Kit (ab102533)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture media, Cell culture supernatant, Other biological fluids, Plasma, Serum, Urine
- Sensitivity: 0.01 mU/well
Overview
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Product name
Alcohol Dehydrogenase Assay Kit
See all Alcohol Dehydrogenase kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Cell culture media -
Assay type
Enzyme activity -
Sensitivity
= 0.01 mU/well -
Assay time
0h 40m -
Product overview
Abcam's Alcohol Dehydrogenase Assay Kit provides a convenient tool for sensitive detection of the Alcohol DH in a variety of samples. In the assay Alcohol DH will utilize isopropanol as a substrate leading to a proportional color development. The activity of ADH can be easily quantified colorimetrically (? = 450 nm). This assay detects ADH activity as low as 0.01 mU in samples.
Visit our FAQs page for tips and troubleshooting.Alcohol dehydrogenase assay protocol summary:
- add samples and standards to wells
- add reaction mix - incubate for 3 min
- analyze with microplate reader, incubate for 30 min to 2 hrs and analyze again -
Notes
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests ADH Assay Buffer WM 1 x 25ml ADH Positive Control (Lyophilized) Green 1 vial Developer (Lyophilized) Red 1 vial Isopropanol Blue 1 x 1ml NADH Standard (Lyophilized) Yellow 1 vial -
Research areas
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Relevance
Alcohol dehydrogenase (Alcohol DH, ADH) (EC 1.1.1.1) is a group of seven dehydrogenase enzymes that occur in many organisms and facilitate the interconversion between alcohols and aldehydes or ketones with the reduction of NAD+ to NADH. In humans and many other animals, they serve to break down alcohols which could otherwise be toxic; in yeast and many bacteria, some alcohol dehydrogenases catalyze the opposite reaction as part of fermentation. -
Cellular localization
Cytoplasmic -
Alternative names
- ADH
- ADH alpha subunit
- ADH beta subunit
see all
Images
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Functional Studies - Alcohol Dehydrogenase Detection Kit (ab102533)
Colorimetric standard curve: mean of duplicates (+/-SD) with background readings subtracted.
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Functional Studies - Alcohol Dehydrogenase Detection Kit (ab102533)Sample: Bovine Liver extraction (36µg protein)