Anti-Siah2 antibody - C-terminal (ab230523)
Key features and details
- Rabbit polyclonal to Siah2 - C-terminal
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Siah2 antibody - C-terminal
See all Siah2 primary antibodies -
Description
Rabbit polyclonal to Siah2 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
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Immunogen
Synthetic peptide corresponding to Human Siah2 (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: O43255 -
Positive control
- IHC-P: Human colon tissue. ICC/IF: MCF7 cells. WB: MCF7 whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Siah2 antibody - C-terminal (ab230523)Anti-Siah2 antibody - C-terminal (ab230523) at 1/500 dilution + MCF7 (Human breast adenocarcinoma cell line) whole cell lysate
Developed using the ECL technique.
Predicted band size: 34 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Siah2 antibody - C-terminal (ab230523)Formalin-fixed, paraffin-embedded human colon tissue stained for Siah2 with ab230523 at a 1/100 dilution in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-Siah2 antibody - C-terminal (ab230523)MCF7 (Human breast adenocarcinoma cell line) cells stained for Siah2 using ab230523 at a dilution of 1/100 in ICC/IF.
Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with ab230523 in 3% BSA-PBS and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight® 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
