Anti-NMDAR2A antibody - N-terminal (ab227233)
Key features and details
- Rabbit polyclonal to NMDAR2A - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR2A antibody - N-terminal
See all NMDAR2A primary antibodies -
Description
Rabbit polyclonal to NMDAR2A - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Chimpanzee
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Immunogen
Recombinant fragment within Human NMDAR2A (N terminal). The exact sequence is proprietary.
Database link: Q12879 -
Positive control
- IHC-P: U87 xenograft tissue. WB: Jurkat whole cell lysate (ab7899); Mouse brain lysate; Rat brain extract. ICC/IF: DIV14 rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution + Rat brain extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 165 kDa5% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody - N-terminal (ab227233)4% paraformaldehyde-fixed DIV14 rat E18 primary cortical neurons stained for NMDAR2A (green) using ab227233 at 1/500 dilution in ICC/IF.
Red: beta Tubulin 3 stained by a beta Tubulin 3 antibody at 1/500 dilution.
Blue: DAPI. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NMDAR2A antibody - N-terminal (ab227233)Paraffin-embedded U87 (human) xenograft tissue stained for NMDAR2A using ab227233 at 1/500 dilution in immunohistochemical analysis.
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Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution + Mouse brain lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 165 kDa5% SDS PAGE gel.
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Western blot - Anti-NMDAR2A antibody - N-terminal (ab227233)Anti-NMDAR2A antibody - N-terminal (ab227233) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 165 kDa5% SDS PAGE gel.
