Anti-GluN2C antibody [EPR19046] (ab192831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19046] to GluN2C
- Suitable for: WB
- Reacts with: Mouse, Rat, Recombinant fragment
Overview
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Product name
Anti-GluN2C antibody [EPR19046]
See all GluN2C primary antibodies -
Description
Rabbit monoclonal [EPR19046] to GluN2C -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatRecombinant fragment -
Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse and rat cerebellum lysates; Mouse GluN2C fragment recombinant protein.
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General notes
Previously labelled as NMDAR2C.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19046 -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-GluN2C antibody [EPR19046] (ab192831) at 1/1000 dilution
Lane 1 : Mouse cerebellum lysate
Lane 2 : Mouse heart lysate
Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate
Lane 4 : Rat liver lysate
Lane 5 : Rat cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1,2,3 and 4: 1 minute; Lane 5: 15 seconds.
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All lanes : Anti-GluN2C antibody [EPR19046] (ab192831) at 1/1000 dilution
Lane 1 : Mouse GluN2C fragment recombinant protein
Lane 2 : Mouse NMDAR2D fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.
Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house. NMDAR2A and NMDAR2B were not tested, as bands of their size (180 kD) were not detected in tissue lysates.
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