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Neuroscience Neurotransmission Receptors / Channels Ligand-Gated Ion Channels NMDA Receptors

Anti-GluN2C antibody [EPR19094] (ab182277)

Price and availability

267 361 ₸

Availability

Order now and get it on Friday March 19, 2021

Anti-GluN2C antibody [EPR19094] (ab182277)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19094] to GluN2C
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human, Recombinant fragment

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Overview

  • Product name

    Anti-GluN2C antibody [EPR19094]
    See all GluN2C primary antibodies
  • Description

    Rabbit monoclonal [EPR19094] to GluN2C
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    Recombinant fragment
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse GluN2C fragment recombinant protein; Mouse and rat cerebellum lysates; Human fetal brain lysate.
  • General notes

    Previously labelled as NMDAR2C.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19094
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • Ligand-Gated Ion Channels
    • NMDA Receptors

Images

  • Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/2000 dilution

    Lane 1 : Mouse GluN2C fragment recombinant protein
    Lane 2 : Mouse NMDAR2D fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane1: 1 second; Lane2: 3 minutes.

    Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution

    Lane 1 : Rat cerebellum lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat liver lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 8987814).

  • Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    Western blot - Anti-GluN2C antibody [EPR19094] (ab182277)
    Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-GluN2C antibody [EPR19094] (ab182277)
    Anti-GluN2C antibody [EPR19094] (ab182277)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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