Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
![Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296742-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg "Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303)
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y]
See all NMDAR2B primary antibodies -
Description
Rabbit monoclonal [EP1858Y] to NMDAR2B (phospho S1303) -
Host species
Rabbit -
Specificity
ab81271 detects NMDAR2B when phosphorylated at serine 1303 -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
A phospho specific peptide corresponding to residues surrounding serine 1303 of human NMDAR2B.
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Positive control
- WB: Human, mouse and rat brain tissue lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1858Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Rat brain tissue lysate
Lane 2 : Rat brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296741-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate. The membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
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![Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-296735-anti-nmdar2b-phospho-s1303-antibody-ep1858y-dot-blot.jpg)
Dot Blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)Dot blot analysis of NMDAR2B (phospho S1303) phospho peptide (Lane 1) and NMDAR2B non-phospho peptide (Lane 2) labelling NMDAR2B (phospho S1303) with ab81271 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution. Blocking buffer: 5% NFDM/TBST. Dilution buffer: 5% NFDM /TBST.
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![Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-96453-anti-nmdar2b-phospho-s1303-antibody-ep1858y-western-blot.jpg)
Western blot - Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)All lanes : Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271) at 1/5000 dilution
Lane 1 : Mouse brain lysates (untreated)
Lane 2 : Mouse brain lysates (treated with AP)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP labelled IgG at 1/2000 dilution
Predicted band size: 166 kDa
Observed band size: 180 kDa why is the actual band size different from the predicted?
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![Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)](https://www.abcam.com/ps/products/81/ab81271/Images/ab81271-5-benefits-of-recombinant-antibodies.png)
Anti-NMDAR2B (phospho S1303) antibody [EP1858Y] (ab81271)
