Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
![Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)](https://www.abcam.com/ps/products/250/ab250609/Images/ab250609-405995-anti-glun2c-antibody-epr19094-bsa-and-azide-free-western-blot.jpg "Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19094] to GluN2C - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-GluN2C antibody [EPR19094] - BSA and Azide free
See all GluN2C primary antibodies -
Description
Rabbit monoclonal [EPR19094] to GluN2C - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab250609 is the carrier-free version of ab182277. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab250609 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19094 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/2000 dilution
Lane 1 : Mouse GluN2C fragment recombinant protein
Lane 2 : Mouse NMDAR2D fragment recombinant protein
Lysates/proteins at 0.01 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDaThis data was developed using ab182277, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.
Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house.
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![Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)](https://www.abcam.com/ps/products/250/ab250609/Images/ab250609-403504-anti-glun2c-antibody-epr19094-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution
Lane 1 : Rat cerebellum lysate
Lane 2 : Mouse cerebellum lysate
Lane 3 : Mouse heart lysate
Lane 4 : Rat liver lysate
Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDa
Exposure time: 30 secondsThis data was developed using ab182277, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
The expression profile is consistent with what has been described in the literature (PMID: 8987814).
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![Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)](https://www.abcam.com/ps/products/250/ab250609/Images/ab250609-399358-anti-glun2c-antibody-epr19094-bsa-and-azide-free-western-blot.jpg)
Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution + Human fetal brain lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 135 kDa
Observed band size: 135 kDa
Exposure time: 30 secondsThis data was developed using ab182277, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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![Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)](https://www.abcam.com/ps/products/250/ab250609/Images/ab250609-4-benefits-of-recombinant-antibodies.png)
Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
