Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19094] to GluN2C - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-GluN2C antibody [EPR19094] - BSA and Azide free
    See all GluN2C primary antibodies

  • Description

    Rabbit monoclonal [EPR19094] to GluN2C - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab250609 is the carrier-free version of ab182277. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab250609 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/2000 dilution

    Lane 1 : Mouse GluN2C fragment recombinant protein
    Lane 2 : Mouse NMDAR2D fragment recombinant protein

    Lysates/proteins at 0.01 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa



    This data was developed using ab182277, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 second; Lane 2: 3 minutes.

    Mouse GluN2C fragment recombinant protein contains aa300-499 with a His-Tag®. NMDAR2D fragment recombinant protein contains aa328-526 with a His-Tag®. These two recombinant proteins were made in-house.

  • Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    All lanes : Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution

    Lane 1 : Rat cerebellum lysate
    Lane 2 : Mouse cerebellum lysate
    Lane 3 : Mouse heart lysate
    Lane 4 : Rat liver lysate
    Lane 5 : Neuro-2a (Mouse neuroblastoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    This data was developed using ab182277, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The expression profile is consistent with what has been described in the literature (PMID: 8987814).

  • Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    Western blot - Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    Anti-GluN2C antibody [EPR19094] (ab182277) at 1/1000 dilution + Human fetal brain lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 135 kDa
    Observed band size: 135 kDa


    Exposure time: 30 seconds


    This data was developed using ab182277, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)
    Anti-GluN2C antibody [EPR19094] - BSA and Azide free (ab250609)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

© 2021 Astana Biomed Group LLP. All rights reserved.