Anti-NMDAR1 antibody - Neuronal Marker (ab17345)
Key features and details
- Rabbit polyclonal to NMDAR1 - Neuronal Marker
- Suitable for: IHC (PFA fixed), WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-NMDAR1 antibody - Neuronal Marker
See all NMDAR1 primary antibodies -
Description
Rabbit polyclonal to NMDAR1 - Neuronal Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC (PFA fixed), WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide corresponding to Rat NMDAR1 aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab17568) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab17345 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes IHC (PFA fixed) Use at an assay dependent concentration.WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).Notes IHC (PFA fixed)
Use at an assay dependent concentration.WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 105 kDa).Target
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Function
NMDA receptor subtype of glutamate-gated ion channels with high calcium permeability and voltage-dependent sensitivity to magnesium. Mediated by glycine. This protein plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. It mediates neuronal functions in glutamate neurotransmission. Is involved in the cell surface targeting of NMDA receptors. -
Sequence similarities
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family. NR1/GRIN1 subfamily. -
Post-translational
modificationsNMDA is probably regulated by C-terminal phosphorylation of an isoform of NR1 by PKC. Dephosphorylated on Ser-897 probably by protein phosphatase 2A (PPP2CB). Its phosphorylated state is influenced by the formation of the NMDAR-PPP2CB complex and the NMDAR channel activity. -
Cellular localization
Cell membrane. Cell junction > synapse > postsynaptic cell membrane. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Enriched in post-synaptic plasma membrane and post-synaptic densities. - Information by UniProt
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Database links
- Entrez Gene: 2902 Human
- Entrez Gene: 14810 Mouse
- Entrez Gene: 24408 Rat
- Omim: 138249 Human
- SwissProt: Q05586 Human
- SwissProt: P35438 Mouse
- SwissProt: P35439 Rat
- Unigene: 558334 Human
see all -
Alternative names
- GluN1 antibody
- Glutamate [NMDA] receptor subunit zeta-1 antibody
- Glutamate receptor ionotropic N methyl D aspartate 1 antibody
see all
Images
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml
Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown). -
Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdomab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected
with NR1 and NR2A recombinant protein at 50 µg
Predicted band size: 105 kDa
Observed band size: ~110 kDa why is the actual band size different from the predicted?HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa). ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (41)
ab17345 has been referenced in 41 publications.
- Pang S et al. Integrin β1/FAK/SRC signal pathway is involved in autism spectrum disorder in Tspan7 knockout rats. Life Sci Alliance 6:N/A (2023). PubMed: 36625203
- Dasdelen MF et al. A Novel Theanine Complex, Mg-L-Theanine Improves Sleep Quality via Regulating Brain Electrochemical Activity. Front Nutr 9:874254 (2022). PubMed: 35449538
- Gong P et al. Electroacupuncture of the trigeminal nerve causes N-methyl-D-aspartate receptors to mediate blood-brain barrier opening and induces neuronal excitatory changes. Front Cell Neurosci 16:1020644 (2022). PubMed: 36313622
- Zhang B et al. Donepezil attenuates the development of morphine tolerance in rats with cancer-induced bone pain: The role of cortical N-methyl-D-aspartate receptors. Neurosci Lett 747:135678 (2021). PubMed: 33516802
- Zhu QM et al. Donepezil prevents morphine tolerance by regulating N-methyl-d-aspartate receptor, protein kinase C and CaM-dependent kinase II expression in rats. Pharmacol Biochem Behav 206:173209 (2021). PubMed: 34058253
Images
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)All lanes : Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml
Lane 1 : HEK293 Whole Cell Lysate Transfected with SHAM
Lane 2 : HEK293 Whole Cell Lysate Transfected with NR1-1a/NR2A
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted?
Additional bands at: 54 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
NMDAR1 has a predicted molecular weight of 105kDa; however it has a number of potential glycosylation and phosphorylation sites which may affect the migration of the protein (SwissProt data). In addition, a band at 37kDa has been observed (data not shown). -
Immunohistochemistry (PFA fixed) - Anti-NMDAR1 antibody - Neuronal Marker (ab17345) This image is courtesy of Sophie Pezet, Univ London Kings Coll, United Kingdom
ab17345 Rabbit polyclonal to NMDAR1 immunofluorescent staining in the rat brain cortex. Primary antibody ab17345 was incubated at room temperature overnight at 0.5µg/ml. Preincubation with the immunizing peptide (ab17568) resulted in complete blocking of the primary antibody staining. Anti rabbit alexa fluor 488 secondary antibody was used at 1/1000 (incubated for 2h at room temperature). Protocol details: rat tissue was intracardially perfusion fixed with 4% PFA followed by an overnight post fixation in the same fixative, cryoprotected in 20% sucrose and frozen in OCT. As expected NMDAR1 staining is observed in the soma, axon and dendrites of neurons.
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Western blot - Anti-NMDAR1 antibody - Neuronal Marker (ab17345)Anti-NMDAR1 antibody - Neuronal Marker (ab17345) at 2 µg/ml + HEK293 cell lysate co-transfected
with NR1 and NR2A recombinant protein at 50 µg
Predicted band size: 105 kDa
Observed band size: ~110 kDa why is the actual band size different from the predicted?HEK293 cells were co-transfected with NMDAR1 (expected MW ~ 105kDa) and NMDAR2A (expected MW ~ 180kDa). ab17345 NMDAR1 antibody specifically recognises NMDAR1 protein and does not recognise NMDAR2A protein. ab17345 was incubated overnight at 4°C at 2µg/ml.
