Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)
![Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)](https://www.abcam.com/ps/products/240/ab240884/Images/ab240884-319577-anti-nmdar2a-antibody-s327-95-n-terminal-immunofluorescence.jpg "Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)")
Key features and details
- Mouse monoclonal [S327-95] to NMDAR2A - N-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
Overview
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Product name
Anti-NMDAR2A antibody [S327-95] - N-terminal
See all NMDAR2A primary antibodies -
Description
Mouse monoclonal [S327-95] to NMDAR2A - N-terminal -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment
Predicted to work with: Mouse, Rat, Chimpanzee
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Immunogen
Fusion protein corresponding to Rat NMDAR2A aa 50-350. (extracellular N-terminus) NP_036705.3.
Database link: Q00959 -
Positive control
- WB: Monkey COS cells transfected with GFP-tagged NMDAR2A. ICC/IF: SK-N-BE cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from TCS. -
Clonality
Monoclonal -
Clone number
S327-95 -
Isotype
IgG2a -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)
Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling NMDAR2A using ab240884 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.
Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.
Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.
(A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240884 (D) Composite.
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![Western blot - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)](https://www.abcam.com/ps/products/240/ab240884/Images/ab240884-319578-anti-nmdar2a-antibody-s327-95-n-terminal-western-blot.jpg)
Western blot - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884) at 1/200 dilution (16 hours at 4°C) + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg
Secondary
Goat Anti-Mouse IgG: HRP for 1 hour RT at 1/1000 dilution
Developed using the ECL technique.
Exposure time: 6 minutesBlock: 2% BSA and 2% skimmed milk in 1X TBST.
