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Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)

Key features and details

  • Mouse monoclonal [S327-95] to NMDAR2A - N-terminal
  • Suitable for: ICC/IF, WB
  • Reacts with: Human, Recombinant fragment
  • Isotype: IgG2a

Overview

  • Product name

    Anti-NMDAR2A antibody [S327-95] - N-terminal
    See all NMDAR2A primary antibodies

  • Description

    Mouse monoclonal [S327-95] to NMDAR2A - N-terminal

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human, Recombinant fragment
    Predicted to work with: Mouse, Rat, Chimpanzee

  • Immunogen

    Fusion protein corresponding to Rat NMDAR2A aa 50-350. (extracellular N-terminus) NP_036705.3.
    Database link: Q00959

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: Monkey COS cells transfected with GFP-tagged NMDAR2A. ICC/IF: SK-N-BE cells.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein G purified

  • Purification notes

    Purified from TCS.

  • Clonality

    Monoclonal

  • Clone number

    S327-95

  • Isotype

    IgG2a

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)
    Immunocytochemistry/ Immunofluorescence - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)

    Immunocytochemistry/Immunofluorescence analysis of SK-N-BE (human neuroblastoma cell line) cells labeling NMDAR2A using ab240884 at 1/100 for 60 minutes at RT. Cells were fixed in 4% formaldehyde for 15 minutes at RT.

    Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1/100 for 60 minutes at RT.

    Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min RT, 5 min RT.

    (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab240884 (D) Composite.

  • Western blot - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)
    Western blot - Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884)
    Anti-NMDAR2A antibody [S327-95] - N-terminal (ab240884) at 1/200 dilution (16 hours at 4°C) + Monkey COS cells transfected with GFP-tagged NMDAR2A at 15 µg

    Secondary
    Goat Anti-Mouse IgG: HRP for 1 hour RT at 1/1000 dilution

    Developed using the ECL technique.

    Exposure time: 6 minutes


    Block: 2% BSA and 2% skimmed milk in 1X TBST.

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