Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸

Order now and get it on Thursday October 13, 2022

Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

Key features and details

Rabbit monoclonal [EP1902Y] to PKC (phospho S660)
Suitable for: Dot blot, IHC-P, WB
Reacts with: Human
Isotype: IgG

Product name

Anti-PKC (phospho S660) antibody [EP1902Y]
See all PKC primary antibodies
Description

Rabbit monoclonal [EP1902Y] to PKC (phospho S660)
Host species

Rabbit
Specificity

ab75837 detects various isoforms of PKC phosphorylated.
Tested applications

Suitable for: Dot blot, IHC-P, WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293 whole cell lysates, untreated or treated with Phorbol-12-myristate-13-acetate, Jurkat whole cell lysate (ab7899), untreated or treated with AP. IHC-P: human breast carcinoma.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1902Y
Isotype

IgG
Research areas
- Neuroscience
- Development
- Neuroscience
- Processes

Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/20000 dilution

Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
Lane 3 : Recombinant human PKC beta 2 protein (ab60841)
Lane 4 : Recombinant human PKC delta protein (ab60844)
Lane 5 : Recombinant human PKC eta protein (ab60849)
Lane 6 : Recombinant human PKC epsilon protein (ab60847)
Lane 7 : Recombinant human PKC theta/PRKCQ protein (ab56641)
Lane 8 : Recombinant human PKC mu/PKD protein (ab60873)

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 100-150 kDa
why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and dilution buffer: 5% NFDM/TBST.

Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag.
Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.
Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag.
Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag.
Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag.
Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag.
Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.
Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/5000 dilution

Lane 1 : HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysates
Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates
Lane 3 : HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa

Exposure time: 3 minutes

Blocking and dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

ab75837, at a 1/100 dilution, staining PKC in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/100000 dilution

Lane 1 : Jurkat cell lysates, untreated
Lane 2 : Jurkat cell lysates, treated with AP

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 77 kDa
Observed band size: 77 kDa
Dot Blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

Dot blot analysis of PKC beta 2 (phospho S660) phospho peptide (Lane 1), PKC beta 2 Non-phospho peptide (Lane 2), labelling PKC (phospho S660) with ab75837 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

Blocking and diluting buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com