Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

Key features and details

  • Rabbit monoclonal [EP1902Y] to PKC (phospho S660)
  • Suitable for: Dot blot, IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-PKC (phospho S660) antibody [EP1902Y]
    See all PKC primary antibodies

  • Description

    Rabbit monoclonal [EP1902Y] to PKC (phospho S660)

  • Host species

    Rabbit

  • Specificity

    ab75837 detects various isoforms of PKC phosphorylated.

  • Tested applications

    Suitable for: Dot blot, IHC-P, WBmore details
    Unsuitable for: Flow Cyt,ICC or IP

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293 whole cell lysates, untreated or treated with Phorbol-12-myristate-13-acetate, Jurkat whole cell lysate (ab7899), untreated or treated with AP. IHC-P: human breast carcinoma.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/20000 dilution

    Lane 1 : Recombinant human PKC alpha protein (Active) (ab55672)
    Lane 2 : Recombinant human PKC beta 1 protein (ab60840)
    Lane 3 : Recombinant human PKC beta 2 protein (ab60841)
    Lane 4 : Recombinant human PKC delta protein (ab60844)
    Lane 5 : Recombinant human PKC eta protein (ab60849)
    Lane 6 : Recombinant human PKC epsilon protein (ab60847)
    Lane 7 : Recombinant human PKC theta/PRKCQ protein (ab56641)
    Lane 8 : Recombinant human PKC mu/PKD protein (ab60873)

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 100-150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    Blocking and dilution buffer: 5% NFDM/TBST.

    Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag.
    Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag.
    Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag.
    Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag.
    Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag.
    Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag.
    Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag.
    Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag.
  • Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/5000 dilution

    Lane 1 : HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysates
    Lane 2 : HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates
    Lane 3 : HEK293 (Human epithelial cell line from embryonic kidney) treated with Phorbol-12-myristate-13-acetate at 100nM for 1 hour whole cell lysates. Then the membrane was incubated with phosphatase.

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa


    Exposure time: 3 minutes


    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

    ab75837, at a 1/100 dilution, staining PKC in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    Western blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    All lanes : Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837) at 1/100000 dilution

    Lane 1 : Jurkat cell lysates, untreated
    Lane 2 : Jurkat cell lysates, treated with AP

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 77 kDa
    Observed band size: 77 kDa

  • Dot Blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)
    Dot Blot - Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

    Dot blot analysis of PKC beta 2 (phospho S660) phospho peptide (Lane 1), PKC beta 2 Non-phospho peptide (Lane 2), labelling PKC (phospho S660) with ab75837 at a dilution of 1/1000. Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/2500.

    Blocking and diluting buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-PKC (phospho S660) antibody [EP1902Y] (ab75837)

© 2021 Astana Biomed Group LLP. All rights reserved.