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Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15353] to MARCKS like protein - C-terminal
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-MARCKS like protein antibody [EPR15353] - C-terminal
    See all MARCKS like protein primary antibodies

  • Description

    Rabbit monoclonal [EPR15353] to MARCKS like protein - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, SH-SY5Y and Human fetal brain lysates; Human colon tissue; 293T and SH-SY5Y cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    All lanes : Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/10000 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : SH-SY5Y cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Immunohistochemical analysis of Paraffin-embedded Human colon tissue labeling MARCKS like protein with ab184546 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Western blot - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546) at 1/50000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 20 kDa



    The protein has a calculated molecular weight of 20 kDa and an apparent molecular weight of 42-48 kDa due to anomalous migration on SDS gels or phosphorylation.

  • Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Immunocytochemistry/ Immunofluorescence - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Immunofluorescent analysis of 293T cells (4% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/250 dilution (red) followed by Goat anti rabbit IgG (AlexaFluor® 555) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Flow Cytometry - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Flow Cytometry - Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

    Flow cytometric analysis of SH-SY5Y cells (2% paraformaldehyde-fixed) labeling MARCKS like protein with ab184546 at 1/140 dilution (red) or a rabbit IgG (negative) (green), followed by Goat anti rabbit IgG (FITC) secondary at 1/150 dilution.

  • Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)
    Anti-MARCKS like protein antibody [EPR15353] - C-terminal (ab184546)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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