Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free
  • Suitable for: IHC-P, ICC, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-SCF antibody [EP665Y] - BSA and Azide free
    See all SCF primary antibodies

  • Description

    Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, ICC, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247320 is the carrier-free version of ab52603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247320 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)

    Secondary
    HRP-labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab52603, the same antibody clone in a different buffer formulation.

  • Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    This data was developed using ab52603, the same antibody clone in a different buffer formulation.Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.

    Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  • Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
    Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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