Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
![Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-407887-anti-scf-antibody-ep665y-bsa-and-azide-free-western-blot.jpg "Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free
- Suitable for: IHC-P, ICC, WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-SCF antibody [EP665Y] - BSA and Azide free
See all SCF primary antibodies -
Description
Rabbit monoclonal [EP665Y] to SCF - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab247320 is the carrier-free version of ab52603. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab247320 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP665Y -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)Anti-SCF antibody [EP665Y] (ab52603) at 1/10000 dilution + recombinant protein (5ng)
Secondary
HRP-labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?This data was developed using ab52603, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-3-anti-scf-antibody-ep665y-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling SCF with purified ab52603 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue). -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-2-anti-scf-antibody-ep665y-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue using ab52603 at a dilution of 1/100-1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-1-anti-scf-antibody-ep665y-bsa-and-azide-free-flow-cytometry.jpg)
Flow Cytometry - Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)This data was developed using ab52603, the same antibody clone in a different buffer formulation.Overlay histogram showing MCF-7 cells stained with ab52603 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52603, 1/20 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF-7 cells fixed with methanol (5 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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![Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)](https://www.abcam.com/ps/products/247/ab247320/Images/ab247320-5-benefits-of-recombinant-antibodies.png)
Anti-SCF antibody [EP665Y] - BSA and Azide free (ab247320)
