Antibodies, Proteins, Kits and Reagents for Life Science

321 638 ₸ Product size

100 µl 321 638 ₸ 10 µl 110 563 ₸

Order now and get it on Tuesday March 09, 2021

Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD
Suitable for: WB, IHC-P, IHC-Fr, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-CEBP Delta/CEBPD antibody [EPR23518-259]
See all CEBP Delta/CEBPD primary antibodies
Description

Rabbit monoclonal [EPR23518-259] to CEBP Delta/CEBPD
Host species

Rabbit

Tested Applications & Species

Application	Species
IHC-Fr	Mouse Rat
IHC-P	Mouse Rat
IP	Mouse
WB	Mouse Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: ,Caco-2, HaCaT and differentiated NIH/3T3 L1 cell lysates. IHC-P: Mouse hippocampus tissue; Rat cerebrum tissue. IHC-Fr: Mouse hippocampus tissue; Rat hippocampus tissue. IP: Differentiated NIH/3T3-L1 cells.
General notes
We cannot guarantee ab245214 in IHC-P or IHC-Fr for human samples. Internal testing demonstrated negative staining in positive control human amygdala.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR23518-259
Isotype

IgG
Research areas

Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

All lanes : Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution

Lane 1 : Caco-2 (human colorectal adenocarcinoma epithelial cell), whole cell lysate
Lane 2 : HaCaT (human skin keratinocyte), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 180 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.
Western blot - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214) at 1/1000 dilution + Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 29 kDa
Observed band size: 29 kDa

Exposure time: 180 seconds

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 180 seconds.
Immunoprecipitation - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

CEBP Delta/CEBPD was immunoprecipitated from 0.35 mg differentiated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate with ab245214 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 245214 1/1000 dilution (0.518 μg/ml). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Differentiated 3T3-L1 (mouse embryonic fibroblast), whole cell lysate 10 μg

Lane 2: ab245214 IP in Differentiated 3T3-L1 whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab245214 in differentiated 3T3-L1 whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 180 seconds.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear and weak cytoplasmic staining on rat cerebrum is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/500 (1.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse hippocampus is observed. The section was incubated with ab245214 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on rat hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse hippocampus tissue labeling CEBP Delta/CEBPD with ab245214 at 1/100 dilution followed by ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution (Green). The nuclear counterstain was DAPI (Blue). Nuclear staining on mouse hippocampus is observed.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor^®488 Goat anti-Rabbit secondary at 1/1000 (2 μg/ml) dilution.
Anti-CEBP Delta/CEBPD antibody [EPR23518-259] (ab245214)

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