Antibodies, Proteins, Kits and Reagents for Life Science

314 937 ₸ Product size

100 µl 314 937 ₸

Order now and get it on Wednesday March 17, 2021

Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1483(N)] to PKC (phospho S729)
Suitable for: Dot blot, WB
Reacts with: Human

Product name

Anti-PKC (phospho S729) antibody [EPR1483(N)]
See all PKC primary antibodies
Description

Rabbit monoclonal [EPR1483(N)] to PKC (phospho S729)
Host species

Rabbit
Specificity

This antibody cross-reacts with several isoforms of PKC.
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Lysate of SH-SY5Y cells treated with Okadaic Acid and Calyculin A.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1483(N)
Isotype

IgG
Research areas

Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - Carrier Free (ab181119)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/2000 dilution

Lane 1 : THP-1 (human monocytic leukemia cell line) whole cell lysate.
Lane 2 : THP-1 whole cell lysate treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 24 hours.
Lane 3 : THP-1 whole cell lysate treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 24 hours. Then the membrane was incubated with phosphatase.

Lysates/proteins at 15 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 84 kDa
Additional bands at: 84 kDa. We are unsure as to the identity of these extra bands.

Exposure time: 3 seconds

Blocking/dilution buffer: 5% NFDM/TSBT
Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - Carrier Free (ab181119)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/1000 dilution

Lane 1 : Active human PKC alpha full length protein (ab55672) contains aa1-672 with GST-tag. at 0.02 µg
Lane 2 : Active human PKC beta 1 full length protein (ab60840) contains aa1-671 with GST-tag. at 0.06 µg
Lane 3 : Active human PKC beta 2 full length protein (ab60841) contains aa1-673 with GST-tag. at 0.02 µg
Lane 4 : Active human PKC delta full length protein (ab60844) contains aa1-676 with GST-tag. at 0.06 µg
Lane 5 : Active human PKC eta full length protein (ab60849) contains aa1-683 with GST-tag. at 0.02 µg
Lane 6 : Active human PKC epsilon full length protein (ab60847) contains aa1-737 with GST-tag. at 0.02 µg
Lane 7 : Active human PKC theta full length protein (ab56641) contains aa1-706 with GST-tag. at 0.02 µg
Lane 8 : Active human PKC mu full length protein (ab60873) contains aa1-912 with GST-tag. at 0.02 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 84 kDa
Additional bands at: 100-150 kDa. We are unsure as to the identity of these extra bands.

Blocking/dilution buffer: 5% NFDM/TBST

Exposure time:

Lanes 1, 3, 5-8: 10 seconds

Lanes 2, 4: 3 minutes
Dot Blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - Carrier Free (ab181119)

Dot blot analysis of PKC epsilon (pS729) phospho peptide (Lane 1), PKC epsilon non-phospho peptide (Lane 2), labelling PKC (pS729) with purified ab181119 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.

Blocking and dilution buffer: 5% NFDM/TBST.

Exposure time: 3 minutes.
Western blot - Anti-PKC (phospho S729) antibody [EPR1483(N)] - Carrier Free (ab181119)

All lanes : Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119) at 1/2000 dilution

Lane 1 : Lysate of SH-SY5Y cells treated with Okadaic Acid and Calyculin A
Lane 2 : Lysate of SH-SY5Y cells

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 84 kDa
Anti-PKC (phospho S729) antibody [EPR1483(N)] (ab181119)

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